A retrospective study was done to evaluate the availability of medical investigation facilities in Holy Family Red Crescent Medical College Hospital (HFRCMCH) for a period of 6 months. The study was designed to find out the gaps in the study Institute which is a tertiary level hospital in Dhaka city. Data was collected from medical records by check list and interview schedule. Out of 49687 patients. 85.28% received services from out patient department and 14.72% from in patient department. Among them. 26151 cases were referred for investigation from out patient and inpatient departments. Out of those 24.36% were blood for routine examination. 17.72% urine for routine examination and 10.77% blood urea and 24.46% blood sugar. 3.82% stool for routine examination and 18.87% for radiological investigation of chest. hi the inpatient department, routine examination of blood was highest (25.38%). next was blood sugar (23.01%). Some specific investigation namely S. Creatinin, S. Electrolytes, Liver function tests, VDRL, widal test, Blood grouping. Culture & sensitivity test were done. Out of specific tests. Blood grouping was highest followed by serum electrolytes. Majority of doctors (86.67) expressed satisfaction in regard to availability of Reagent and Films. Most doctors (93.33) expressed satisfactions in regard to result of laboratory tests and imaging. Regarding the skill of laboratory technician, 43.33% doctors opined good and 56.67% opined as moderate. The results of the study might help the administrator and planner to take steps for further improvement and appropriate utilization of laboratory facilities. However, there is an urgent need for improvement and horizon of investigation facilities should be expanded.