Countries of South East Asia Region have high prevalence rates of viral hepatitis. Hepatitis B is a major public health problem of the region, although it varies widely among the countries. In the region, every year approximately 14-16 million people are infected with Hepatitis B Pilaus (HBO. It is estimated that there are 78 million HBV carriers, which is almost 6% of the total population of the region (1.4 billion, 1995). A cross sectional descriptive study conducted by face to face interview on the basis of a structured questionnaire to identify the selected Dentists in Dhaka city from January to June 2010 among the dentists of City Dental College Hospital and Pioneer Dental College Hospital. Total 120 dentists were included purposively from two hospital in Dhaka city. The study was done to determine the proportion of Hepatitis B vaccination among dentists in a selected hospitals, the history of exposure of hepatitis B among the dentist, why a group of dentist could not take hepatitis B vaccine. Among 120 respondents, most of them received vaccine (65.0 %) whereas 35.0% did not take any. Among 79 respondents, 48.1% (38) finished taking the vaccines whereas 51.9% (41) did not finish taking the vaccines. Negligence was found to be the major cause for not taking the vaccine and very few of them think that the prevalence of hepatitis B is high among dentist. Further in depth study could help investigate to explain role of these factors. All health care personnel should be trained up so that they can save themselves and can also motivate their clients, relatives and general population. Time to time special training is orientation courses should be offered to the dentist for improvement of knowledge regarding hepatitis B infection.