Introduction: The choice of anesthetic agents during oocyte pick-up (OPU) for in vitro fertilization (IVF) mayinfluence various IVF outcome parameters. This study aimed to investigate the impact of different anestheticagents on the outcomes of IVF. Methods: This retrospective observational study was conducted using hospitalrecords from December 2021to March 2023. A total of 200 participants were divided into three groups: GroupA (n=110) received propofol, Group B (n=50) received ketamine, and Group C (n=40) received a combinationof both. Baseline demographics, hormone levels, infertility causes, ovarian stimulation, anesthesia-relatedparameters, oocyte retrieval, embryo quality, and IVF success rates were compared. Result: The study comparedthree groups of participants undergoing IVF with different anesthetics: Group A (n=110) with propofol, GroupB (n=50) with ketamine, and Group C (n=40) with a combination of propofol and ketamine. Participants’demographics, infertility causes, and hormonal levels showed no significant differences among the groups.Anesthesia-related parameters were also similar. Although MI oocytes were significantly higher in Group B(p=0.011), other oocyte and embryo parameters were comparable across the groups. The fertilization ratewas significantly different (p=0.005), with Group A at 54.65%, Group B at 40.49%, and Group C at 59.62%.However, implantation, clinical pregnancy, and take-home baby rates showed no significant differencesamong the groups. Conclusion: The choice of anesthetic agents during oocyte pick-up has minimal impact onmost in vitro fertilization outcome parameters. While a few specific factors, such as metaphase I oocytes andfertilization rates, were influenced by the choice of anesthetic, further large-scale, prospective, randomizedstudies are needed to confirm these findings and optimize IVF success rates while ensuring patient comfortand safety.